|
KMID : 0545120050150020368
|
|
Journal of Microbiology and Biotechnology
2005 Volume.15 No. 2 p.368 ~ p.375
|
|
|
Exo-Polysaccharide Production in Liquid Culture of Pleurotus ferulae
|
|
Choi DB
Kang SH/Song YH/Kwun KH/Jeong KJ/Cha WS
|
|
|
Abstract
|
|
|
|
Batch cultures were carried out to optimize the exo-polysaccharide production by liquid cultures of Pleurotus ferulae. Among the various carbon sources when 5% of glucose was used the maximum mycelial growth and exopolysaccharide concentration reached were 8.78 g/l and 3.59 g/l respectively. Yeast extract and polypeptone were identified as the most suitable nitrogen sources. In particular when a mixture of 1% of polypeptone and 0.8% of yeast extract was used 9.52 g/l of mycelial growth and 4.09 g/l of exopolysaccharide were obtained. In the case of mineral sources K2HPO4 and MgSO4·7H2O were found to be the best mineral sources for mycelial growth and exo-polysaccharide production. Under the optimized culture conditions the agitation speed and aeration were investigated for mycelial growth and exopolysaccharide production in a jar fermentor. The maximum mycelial growth and exo-polysaccharide concentration at 1.5 vvm and 200 rpm obtained were 13.2 g/l and 4.95 g/l respectively after 10 days of culture which were 76% and 79% higher than those of the basal medium. The specific growth rate was decreased with the increase of mycelial growth. However the specific production rate of the exo-polysaccharide was proportionally increased with the specific growth rate. The proposed model profiles showed good agreement with the experimental results for the mycelial growth and exo-polysaccharide production. The specific production rate using the optimized medium was higher than that of basal medium.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|